Javad Mirnajafi-zade; Ali Gorji; Yaghoob Fathollahi; Mahyar Janahmadi; Parviz shahabi; Hadi Kazemi
Volume 18, Issue 3 , September and October 2011, , Pages 148-157
Abstract
Background and Purpose: Spreading depression (SD) is a neuronal-glyal depolarization، with a possible role in different neurological disorders including epilepsy and migraine aura. Initiation and propagation of SD modulate excitability of neuronal network. The aim of the present study was to investigate ...
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Background and Purpose: Spreading depression (SD) is a neuronal-glyal depolarization، with a possible role in different neurological disorders including epilepsy and migraine aura. Initiation and propagation of SD modulate excitability of neuronal network. The aim of the present study was to investigate electrophysiological characteristics of neurons of the lateral amygdala in the late phase of excitability during SD. Methods and Materials: In this experimental study، 6 male rats were used. We used horizontal amygdala-hippocampus-neocortex slice in which SD was induced by KCl application in each brain structure. After superfusion of these slices with GABAA receptor antagonist bicuculline (1.25 µmol/L) for 45 min، initiation of SD evoked ictal epileptic activity in all tested slices. The induction of SD in the lateral amygdala resulted in presence of interictal and ictal epileptiform field potentials and intracellular paroxysmal shift (PDS). For data analysis، paired t-test and one-way ANOVA were used in Sigma Stat 3 software. Results: The results demonstrated that SD moved the resting membrane potential (before -60.3±0.5 and after -52.8±0.78) towards depolarization after inducing the spreading depression in lateral Amygdale (P